Vaccination of chickens one to fourteen days old against Newcastle poultry disease, strain Bio 52: NDV B1 DOSAGE AND ADMINISTRATION Day-old chickens can be vaccinated by spraying using spray cabinet. The vaccine can also be applied into the nostril or by eye drop. For the eye and nose droplets, the vaccine should be diluted in purified distilled water or water for injection. Another way of mass vaccination is application via fresh drinking water.